GM14193
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 4 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a human del(4)(p16.2) in 88% of cells & a #5 in 96%
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line HHW832; produced by the fusion of human cells with Chinese hamster cells; grown in medium with 0.2mM proline at 39C; retains a human del(4)(p16.2) in ??% of cells & other human chromosomes |
| Jiang G, Yang F, Van Overveld PG, Vedanarayanan V, Van Der Maarel S, Ehrlich M, Testing the Position-Effect Variegation Hypothesis for Facioscapulohumeral Muscular Dystrophy By Analysis of Histone Modification and Gene Expression in Subtelomeric 4q. Hum Mol GenetEpub ahead of print: 2003 |
| PubMed ID: 14506132 |
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| Smith B, Skarecky D, Bengtsson U, Magenis RE, Carpenter N, Wasmuth JJ, Isolation of DNA markers in the direction of the Huntington disease gene from the G8 locus. Am J Hum Genet42:335-44 1988 |
| PubMed ID: 2963539 |
| Split Ratio |
1:20 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 34.5mg/L |
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