GM14194
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 4 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/Chinese hamster hybrid retaining a human der(4)t(4;21)(p16.1;q21.1) in 92% of cells and other human chromos
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line HHW892; subclone of HHW847; produced by the fusion of human cells & Chinese hamster cells; grown in medium with 0.2mM proline at 39C; retains a human del(4)t(4;21)(p16.1;q21.1) in ??% of cells & other human chromosomes |
| Wijmenga C, Winokur ST, Padberg GW, Skraastad MI, Altherr MR, Wasmuth JJ, Murray JC, Hofker MH, Frants RR, The human skeletal muscle adenine nucleotide translocator gene maps to chromosome 4q35 in the region of the facioscapulohumeral muscular dystrophy locus. Hum Genet92:198-203 1993 |
| PubMed ID: 8103757 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 34.5mg/L |
|
|