GM14196
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 5 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
Human/Chinese hamster hybrid retaining a human del(5)(p15.2p15.3) in 100% of cells, #13 in 64%, & #14 in 60%
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
Line HHW750; produced by the fusion of human cells with Chinese hamster cells; grown in culture medium with 0.2mM proline at 39C; retains a human del(5) (p15.2p15.3) in ??% of cells & other human chromosomes |
| Church DM, Bengtsson U, Nielsen KV, Wasmuth JJ, Niebuhr E, Molecular definition of deletions of different segments of distal 5p that result in distinct phenotypic features. Am J Hum Genet56:1162-1172 1995 |
| PubMed ID: 7726173 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 34.5mg/L |
|
|