GM14231
Somatic cell hybrid
Description:
CHROMOSOME 5 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/Chinese hamster hybrid retaining a human der(22)t(5;22)(q11.2;q13.3) in 95% of cells and #4,11,13,14,15,20, & 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
PCR analysis of DNA from this somatic cell hybrid gave negative results with primers for 5p14-p12,C9, and 5p15.3-p15.1, D5S208, and gave positive results with primers for 5q11.2-q13.1, D5S351, 5q13.3-q14, HMGCR, 5q32, D5S372, and 5q34-q35, GABRA1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 22 |
PCR analysis of DNA from this somatic cell hybrid gave a positive result with a primer for 22q12.1-q13.1, D22S300, and gave negative results with primers for 22q13.31-qter, DIA1, and 22q13.31, KI-108. |
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| Remarks |
Line HHW1405; produced by the fusion of human cells, GM10308, with Chinese hamster cells; grown in medium with 0.2mM proline at 39C; retains a human der(22) t(5;22)(q11.1;q13.3) in ??% of cells & other human chromosomes |
| Abrams WR, Ma RI, Kucich U, Bashir MM, Decker S, Tsipouras P, McPherson JD, Wasmuth JJ, Rosenbloom J, Molecular cloning of the microfibrillar protein MFAP3 and assignment of the gene to human chromosome 5q32-q33.2. Genomics26:47-54 1995 |
| PubMed ID: 7782085 |
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| McPherson JD, Morton RA, Ewing CM, Wasmuth JJ, Overhauser J, Nagafuchi A, Tsukita S, Isaacs WB, Assignment of the human alpha-catenin gene (CTNNA1) to chromosome 5q21- q22. Genomics19:188-90 1994 |
| PubMed ID: 8188230 |
| Split Ratio |
1:5 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 34.5mg/L |
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