GM14236
LCL from B-Lymphocyte
Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46,XY,t(5;9) (5pter>5q32::9p24>9pter; 9qter>9p24::5q32>5qter), t(1;14)(1qter>1p36.1::14q32.3>14qter;14pter>14q32.3::1p36.1>1pter)[32]/46,XY,t(5;9)(5pter>5q32::9p24>9pter; 9qter>9p24::5q32>5qter)[18]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Cytogenetics |
Chromosome 1: TRANSLOCATION Breakpoint 1p36 t(1;14)1p36 |
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Chromosome 14: TRANSLOCATION Breakpoint 14q32 t(1;14)14q32 |
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Chromosome 5: TRANSLOCATION Breakpoint 5q32 t(5;9)5q32 |
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Chromosome 9: TRANSLOCATION Breakpoint 9p24 t(5;9)9p24 |
| Remarks |
Line 1594; 46,XY,t(5;9)(5pter>5q32:: 9p24>9pter;9qter>9p24::5q32>5qter/46,XY, t(5;9)(q32;p24),t(1;14)(p36.1;q32.3); balanced; 36%/64% & 2% of cells show random chromosome gain |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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