GM14853
Somatic cell hybrid
Description:
CHROMOSOME 1 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse hybrid retaining a human der(17)t(1;17)(p34;p13) in 92% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line 20L-17; produced by fusing human fibro, GM00201A, 47,XX,+21,t(1;17) (1qter>1p34::17p13>17pter;17qter>17p13:: 1p34>1pter), with LMTK- mouse cells; grown in HAT medium; 92% of cells retain a human der(17)t(1;17)(p34;p13) |
| Schweickart VL, Raport CJ, Godiska R, Byers MG, Eddy RL Jr, Shows TB, Gray PW, Cloning of human and mouse EBI1, a lymphoid-specific G-protein-coupled receptor encoded on human chromosome 17q12-q21.2. Genomics23:643-50 1994 |
| PubMed ID: 7851893 |
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| Backer JM, Mendola CE, Kovesdi I, Fairhurst JL, O'Hara B, Eddy RL Jr, Shows TB, Mathew S, Murty VV, Chaganti RS, Chromosomal localization and nucleoside diphosphate kinase activity of human metastasis-suppressor genes NM23-1 and NM23-2. Oncogene8:497-502 1993 |
| PubMed ID: 8381224 |
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| Hautala T, Byers MG, Eddy RL, Shows TB, Kivirikko KI, Myllyla R, Cloning of human lysyl hydroxylase: complete cDNA-derived amino acid sequence and assignment of the gene (PLOD) to chromosome 1p36.3---- p36.2. Genomics13:62-9 1992 |
| PubMed ID: 1577494 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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