GM14954
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 16 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/hamster somatic cell hybrid retaining a human der(11)t(11;16)(q14;q12.1) in 96% of cells and other human chromosomes
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line CY8; produced by the fusion of human cells, der(11)t(11;16)(q14;q12), with HPRT- and APRT-deficient mouse cell line A9; grown in culture medium containing adenine and azaserine; retains a human der(11)t(11;16)(q14;q12.1) in 96% of the cells and human chromosomes 5, 7, 8, 20, and 21; a human chromosome 3 may be present in very low frequency |
| Richards RI, Holman K, Lane S, Sutherland GR, Callen DF, Human chromosome 16 physical map: mapping of somatic cell hybrids using multiplex PCR deletion analysis of sequence tagged sites. Genomics10(4):1047-52 1991 |
| PubMed ID: 1916811 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 5 x 10-2mM |
| Supplement |
Azaserine 5 x 10-2mM |
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