GM14971
Somatic cell hybrid
Description:
CHROMOSOME 11 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 16 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(16)t(11;16)(q21;p12.3) in 56% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line CY11; produced by the fusion of human fibros, GM03005, 46,XX,t(11;16)(q13;p11), with HPRT- and APRT-deficient mouse A9 cells; culture is grown in medium containing adenine and azaserine; retains a human der(16)t(11;16)(q21;p12.3) in 56% of cells; this hybrid tends to form human/rodent translocations and users who elect to use the culture rather than the DNA sample should monitor the culture for retention of the der(16) |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 5 x 10-2mM |
| Supplement |
Azaserine 5 x 10-2mM |
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