GM14976
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 16 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
Human/mouse somatic cell hybrid retaining a human der(16)t(11;16)(q23;p12) in 96% of cells and other human chromosomes
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line CY165; produced by the fusion human cells, der(16)t(11;16)(q23;p12.1>.2), with APRT deficient mouse A9 cells; retains a human der(16)t(11;16)(q23;p12) in 96% of cells and other human chromosomes |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 5 x 10-2mM |
| Supplement |
Azaserine 5 x 10-2mM |
|
|