GM15296
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 7 SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 6 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(6)t(6;7)(q27;q22) in 44% of cells and a 4, 5, 14, 16, & X
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line 2068RAG22-2; produced by the fusion of human fibroblast line GM02068, 46,XX,t(6;7)(6pter>6q27::7q22>7qter;7pter>7q22::6q27>6qter), with RAG mouse cells; retains a human der(6)t(6;7)(q27;q22) in 44% of cells and other human chromosomes |
| Grzeschik KH, Tsui LC, Green ED, Report and abstracts of the First International Workshop on Human Chromosome 7 Mapping 1993 Cytogenetics and cell genetics65:52-73 1994 |
| PubMed ID: 8104767 |
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| Rommens JM, Zengerling S, Burns J, Melmer G, Kerem BS, Plavsic N, Zsiga M, Kennedy D, Markiewicz D, Rozmahel R, et al, Identification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene. Am J Hum Genet43:645-63 1988 |
| PubMed ID: 2903665 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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