GM15301
Somatic cell hybrid
Description:
CHROMOSOME 7 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 6 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(7)t(6;7)(q27;p13) in 80% of cells and 1, 3, 14, 15, 16, 19, 20, and 21
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Produced by the fusion of human fibroblasts, 46,XX,t(6;7)(q27;p13), with RAG mouse cells; culture grown without selection; retains a human der(7)t(6;7)(q27;p13) in 80% of cells and other human chromosomes |
| Moore MW, Dietz LG, Tirtorahardjo B, Cotter PD, A multiplex methylation PCR assay for identification of uniparental disomy of chromosome 7 Human mutation21:645-8 2003 |
| PubMed ID: 12754712 |
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| Vortkamp A, Thias U, Gessler M, Rosenkranz W, Kroisel PM, Tommerup N, Kruger G, Gotz J, Pelz L, Grzeschik KH, A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p. Genomics11:737-43 1991 |
| PubMed ID: 1663489 |
| Split Ratio |
1:9 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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