GM15452
Fibroblast from Gonad, Ovary
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Ovary
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Cell Type
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Fibroblast
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Tissue Type
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Gonad
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Gonad, Ovary
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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Aneuploid Chinese hamster ovary line CHO-K1 n=21 range 19-23
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| Remarks |
Chinese hamster ovary line CHO-K1 |
| Sturgeon CM, Knight ZA, Shokat KM, Roberge M, Effect of combined DNA repair inhibition and G2 checkpoint inhibition on cell cycle progression after DNA damage Molecular cancer therapeutics5:885-92 2006 |
| PubMed ID: 16648558 |
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| Kao FT, Puck TT, Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells. Proc Natl Acad Sci U S A60(4):1275-81 1968 |
| PubMed ID: 5244736 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
5% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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