GM15827
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/CHINESE HAMSTER SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/hamster somatic cell hybrid retaining a human chromosome 11 in 100% of cells
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Remarks
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| Remarks |
Line AND6; produced by the fusion of human lymphoblasts with Chinese hamster ovary line CHOK1; grown in culture medium with 750ug/ml geneticin (G418); this hybrid retains a human chromosome 11 in 100% of cells. |
| Kraemer SM, Waldren CA, Chromosomal mutations and chromosome loss measured in a new human-hamster hybrid cell line, ALC: studies with colcemid, ultraviolet irradiation, and 137Cs gamma-rays. Mutat Res379(2):151-66 1997 |
| PubMed ID: 9357544 |
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| Ueno AM, Vannais DB, Gustafson DL, Wong JC, Waldren CA, A low, adaptive dose of gamma-rays reduced the number and altered the spectrum of S1- mutants in human-hamster hybrid AL cells. Mutat Res358(2):161-9 1996 |
| PubMed ID: 8946021 |
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| Waldren C, Braaton M, Vannais D, Fouladi B, Parker RD, The use of human repetitive DNA to target selectable markers into only the human chromosome of a human-hamster hybrid cell line (AL). Somat Cell Mol Genet18(5):417-22 1992 |
| PubMed ID: 1475708 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
7% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Geneticin 750ug/ml |
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