GM15859
Fibroblast from Gonad, Ovary
Description:
DICENTRIC CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Ovary
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Cell Type
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Fibroblast
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Tissue Type
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Gonad
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Transformant
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Simian Virus 40
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Sample Source
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Fibroblast from Gonad, Ovary
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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44-49,X,dic(X)(pter>q28::q28>pter),+markers[cp10]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
SV40-transformed GM07213 Fibroblast [46,X,dic(X)(pter>q28::q28>pter)]; Culture established from ovarian biopsy; small infantile uterus and cervix; undergoing chemotherapy for previous malignancy; dic(X) is late labelling |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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