Description:
ANEUPLOID CHROMOSOME NUMBER - NON-TRISOMIC
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities
Engineered Cell Cultures |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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45,XY,der(11)t(1;11)(11qter>11p15::1q12>1qter),der(12;14)(12pter>12p10::14q10>14qter),der(12;15)(12qter>12q10::15q10>15qter).rev ish enh(1q12>1qter)[9]/del(10)(p11),der(11)t(1;11)(11qter>11p15
::1q12>1qter),der(12;14)(12pter>12p10::14q10>14qter),der(12;15)(12qter>12q10::15q10>15qter).rev ish enh(1q12>1qter)[1]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
HPRT- mutant derived from line MSU1.1, transfected with pPMH16 plasmid containing the bacterial gpt gene integrated into human chromosomes 1, 4, & 10; selected with HAT medium; mean chromosome number is 45 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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