GM15923
Tumor-derived cell line from Bladder carcinoma
Description:
ANEUPLOID CHROMOSOME NUMBER - NON-TRISOMIC
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities
Engineered Cell Cultures |
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Biopsy Source
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Bladder carcinoma
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Cell Type
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Tumor-derived cell line
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Tissue Type
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Tumor
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Sample Source
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Tumor-derived cell line from Bladder carcinoma
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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45,X,del(4)(q31q33),i(5)(q10),der(8)t(7;8)(q32;p12),del(9)(p21),der(10)t(10;22)(p11;q12),t(15;18)(q21;p11),add(18)(p11.2),+der(20)t(11;20)(p11;p11),-21,-22,del(22)(q12).rev ish dim(Y),dim(4)(q31q33),dim(8p),dim(9p),dim(10p),enh(11p),enh(20q),dim(21)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Subclone of line GM16260; transfected with pPMH16 containing the bacterial gpt gene integrated into a human der(11); GM15923 is monosomic for 4q31>4q33, 8p, 9p, 10p, and 21 with no Y present |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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