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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Gonad
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Genbank |
COX1 |
| Remarks |
Chinese hamster ovary-derived fibroblast cell line CHO-9, a subclone of the CHO-KK cell line described by Burki et al (Mut Res 69:347-356,1980). CHO-9 has normal sensitivity to UVB radiation and was the parental cell line for a series of DNA repair deficient mutant cell lines, including: CHO 43-3B (Wood and Burki Mut Res 95:505-514, 1982), the ERCC1 -/- excision repair defective cell line (van Duin et al Cell 44:913-923, 1986), and the wild type parent of EM-C11, the XRCC1 mutant cell line (GM16133), and of XR-C1, the XRCC7 mutant cell line (GM16135). |
| Wood RD, Burki HJ, Repair capability and the cellular age response for killing and mutation
induction after UV. Mutat Res95(2-3):505-14 1982 |
| PubMed ID: 6889678 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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