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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Gonad
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Genbank |
COX1 |
| Remarks |
Chinese hamster ovary mutant cell line XR-C1, derived from CHO-9 fibroblast cells (GM16132) by ethylnitrosourea mutageneis. XR-C1 is defective in DNA double strand break repair and V(D)J recombination. XR-C1 belongs to x-ray complementation group 7 and is defective in the catalytic subunit of DNA dependent protein kinase (DNA-PKcs). |
| Zdzienicka MZ, Mammalian X-ray-sensitive mutants which are defective in non-homologous
(illegitimate) DNA double-strand break repair. Biochimie81(1-2):107-16 1999 |
| PubMed ID: 10214915 |
| |
| Errami A, He DM, Friedl AA, Overkamp WJ, Morolli B, Hendrickson EA,
Eckardt-Schupp F, Oshimura M, Lohman PH, Jackson SP, Zdzienicka MZ, XR-C1, a new CHO cell mutant which is defective in DNA-PKcs, is impaired in both
V(D)J coding and signal joint formation. Nucleic Acids Res26(13):3146-53 1998 |
| PubMed ID: 9628911 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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