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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Genbank |
COX1 |
| Remarks |
Chinese hamster lung mutant cell line V-B11, derived from V79 (GM16136) by ethylnitrosourea-induced mutagenesis. The cell line is UV sensitive (D10 for survival is 6J/m2 compared with 15J/m2 for the V79 parent). V-B11 is the only representative of complemenation group 7 of the UV-sensitive rodent cell mutants. |
| Reardon JT, Thompson LH, Sancar A, Rodent UV-sensitive mutant cell lines in complementation groups 6-10 have normal
general excision repair activity. Nucleic Acids Res25(5):1015-21 1997 |
| PubMed ID: 9023113 |
| |
| Zdzienicka MZ, Mitchell DL, Venema J, van Hoffen A, van Zeeland AA,
Mullenders LH, de Wit J, Simons JW, DNA repair characteristics and mutability of the UV-sensitive V79 Chinese
hamster cell mutant V-B11 (complementation group 7). Mutagenesis6(3):179-83 1991 |
| PubMed ID: 1652676 |
| |
| Zdzienicka MZ, van der Schans GP, Simons JW, Identification of a new seventh complementation group of UV-sensitive mutants in
Chinese hamster cells. Mutat Res194(2):165-70 1988 |
| PubMed ID: 3412364 |
| |
| Zdzienicka MZ, Simons JW., Mutagen-sensitive cell lines are obtained with a high frequency in V79 Chinese
hamster cells. Mutat Res.178(2):235-44 1987 |
| PubMed ID: 87228814 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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