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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Chinese hamster lung mutant cell line V-G8, derived from V79 (GM16136) by ethylnitrosourea-induced mutagenesis. V-G8 is unusually sensitive to killing by x-ray, bleomycin, and methyl methane sulfonate. It belongs to x-ray sensitive complementation group 8 and shows normal repair of DNA single and double strand breaks. V-G8 shows radioresistant DNA synthesis similar to that of human ataxia-telangiectasia cell lines. |
| Chailapakul P, Maeda J, Kato TA, ATM dysfunction in Chinese hamster XRCC8 mutants Biochemical and biophysical research communications736:150491 2024 |
| PubMed ID: 39142236 |
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| Maeda J, Haskins JS, Kato TA, XRCC8 mutation causes hypersensitivity to PARP inhibition without Homologous recombination repair deficiency Mutation research826:111815 2022 |
| PubMed ID: 36812659 |
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| Jongmans W, Verhaegh GW, Jaspers NG, Demant P, Natarajan AT, Shiloh Y,
Oshimura M, Stanbridge EJ, Athwal RS, Cuthbert AP, Newbold RF, Lohman PH,
Zdzienicka MZ, The defect in the AT-like hamster cell mutants is complemented by mouse
chromosome 9 but not by any of the human chromosomes. Mutat Res364(2):91-102 1996 |
| PubMed ID: 8879275 |
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| Jongmans W, Verhaegh GW, Sankaranarayanan K, Lohman PH, Zdzienicka MZ, Cellular characteristics of Chinese hamster cell mutants resembling ataxia
telangiectasia cells. Mutat Res294(3):207-14 1993 |
| PubMed ID: 7692260 |
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| Verhaegh GW, Jaspers NG, Lohman PH, Zdzienicka MZ, Co-dominance of radioresistant DNA synthesis in a group of AT-like Chinese
hamster cell mutants. Cytogenet Cell Genet63(3):176-80 1993 |
| PubMed ID: 8485992 |
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| Zdzienicka MZ, Jaspers NG, van der Schans GP, Natarajan AT, Simons JW, Ataxia-telangiectasia-like Chinese hamster V79 cell mutants with radioresistant
DNA synthesis, chromosomal instability, and normal DNA strand break repair. Cancer Res49(6):1481-5 1989 |
| PubMed ID: 2466555 |
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| Zdzienicka MZ, Simons JW, Mutagen-sensitive cell lines are obtained with a high frequency in V79 Chinese
hamster cells. Mutat Res178(2):235-44 1987 |
| PubMed ID: 3587254 |
| Split Ratio |
1:12 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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