|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Animals |
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Lung
|
|
Transformant
|
Untransformed
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Cricetulus griseus
|
|
Common Name
|
chinese hamster
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Chinese hamster lung cell line XR-V15B was derived by ethylnitrosourea-induced mutagenesis of V79B fibroblasts (GM16143). XR-V15B is hypersensitive to x-irradiation and belongs to complementation group 5 of x-ray sensitive rodent cell mutants. XR-V15B is mutated in the Ku80 subunit of the Ku autoantigen and is defective in non-homologous (illegitimate) DNA double strand break repair and in V(D)J recombination. |
| Zdzienicka MZ, Mammalian X-ray-sensitive mutants which are defective in non-homologous
(illegitimate) DNA double-strand break repair. Biochimie81(1-2):107-16 1999 |
| PubMed ID: 10214915 |
| |
| Errami A, Smider V, Rathmell WK, He DM, Hendrickson EA, Zdzienicka MZ, Chu G, Ku86 defines the genetic defect and restores X-ray resistance and V(D)J recombination to complementation group 5 hamster cell mutants Molecular and cellular biology16:1519-26 1996 |
| PubMed ID: 8657125 |
| |
| Zdzienicka MZ, Mammalian mutants defective in the response to ionizing radiation-induced DNA
damage. Mutat Res336(3):203-13 1995 |
| PubMed ID: 7739608 |
| |
| Rathmell WK, Chu G., Involvement of the Ku autoantigen in the cellular response to DNA double-strand
breaks. Proc Natl Acad Sci U S A91(16):7623-7 1994 |
| PubMed ID: 8052631 |
| |
| Smider V, Rathmell WK, Lieber MR, Chu G, Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku
cDNA. Science266(5183):288-91 1994 |
| PubMed ID: 7939667 |
| |
| Pergola F, Zdzienicka MZ, Lieber MR, V(D)J recombination in mammalian cell mutants defective in DNA double-strand
break repair. Mol Cell Biol13(6):3464-71 1993 |
| PubMed ID: 8497262 |
| |
| Zdzienicka MZ, Tran Q, van der Schans GP, Simons JW, Characterization of an X-ray-hypersensitive mutant of V79 Chinese hamster cells. Mutat Res194(3):239-49 1988 |
| PubMed ID: 2460764 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|