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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Cricetulus griseus
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Common Name
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chinese hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line XR-V16B; an x-ray sensitive Chinese hamster cell line which was derived from V79B cells after treating the cells with an ethylnitrosourea (ENU) and then selected by replica plating as radiosensitive; belongs to x-ray complementation group 5 and is mutated in Ku80 of the Ku autoantigen; defective in DNA double-strand break repair and V(D)J recombination |
| Zdzienicka MZ, Mammalian X-ray-sensitive mutants which are defective in non-homologous
(illegitimate) DNA double-strand break repair. Biochimie81(1-2):107-16 1999 |
| PubMed ID: 10214915 |
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| Errami A, Finnie NJ, Morolli B, Jackson SP, Lohman PH, Zdzienicka MZ, Molecular and biochemical characterization of new X-ray-sensitive hamster cell
mutants defective in Ku80. Nucleic Acids Res26(19):4332-8 1998 |
| PubMed ID: 9742232 |
| Split Ratio |
1:7 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F10 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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