Description:
ANEUPLOID CHROMOSOME NUMBER - NON-TRISOMIC
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities
Engineered Cell Cultures |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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43,XY, der(1)(13qter>13q12::1p34>1qter),-4, del(10)(:p11.1>qter),der(11)(1qter>1q12::11p15>11qter),
der(12)(15qter>15q10::12p10>12qter),-13,der(14)(12pter>12p10::14p10>14qter,-15[5]/
44,XY, der(1)(13qter>13q12::1p34>1qter),-4,del(9)(:p13>qter),del(10)(:p11.1>qter),der(11)(1qter>1q12::11p15>11qter),
der(12)(15qter>15q10::12p10>12qter),-13,der(14)(12pter>12p10::14p10>14qter,-15,+mar[5]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
34 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
A monosomic segregant of line GM15916; GM16262 is monosomic for 1pter>1p32, 4, 10p and 13q12 |
| Passage Frozen |
34 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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