GM16439
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 1 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/hamster somatic cell hybrid retaining a human der(18)t(1;18) in 96% of cells and other human chromosomes
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line JH556; produced by the fusion of human cell line GM16438 (JL480) with karyotype 46,XY,t(1;18)(q32.1q22.1), with Chinese hamster cell line UCW206; grown at 39C; retains a human der(18)t(1;18)(q32.1q22) in 96% of the cells and other human chromosomes |
| Frizell ER, Sutphen R, Diamond FB Jr, Sherwood M, Overhauser J, t(1;18)(q32.1;q22.1) associated with genitourinary malformations. Clin Genet54(4):330-3 1998 |
| PubMed ID: 9831345 |
| Split Ratio |
1:9 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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