GM16444
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/hamster somatic cell hybrid retaining a human del(18)(q21.3) in 100% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line JH478; produced by the fusion of human cell line GM16443 (JL320) with karyotype 46,XY,del(18)(q21.3), with Chinese hamster cell line UCW206; grown at 39C; retains a human del(18)(q21.3) in 100% of the cells |
| Maeda J, Haskins JS, Kato TA, XRCC8 mutation causes hypersensitivity to PARP inhibition without Homologous recombination repair deficiency Mutation research826:111815 2022 |
| PubMed ID: 36812659 |
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| Pellegrino JE, Schnur RE, Boghosian-Sell L, Strathdee G, Overhauser J, Spinner NB, Stump T, Grace K, Zackai EH, Ablepharon macrostomia syndrome with associated cutis laxa: possible localization to 18q. Hum Genet97(4):532-6 1996 |
| PubMed ID: 8834257 |
| Split Ratio |
1:7 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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