GM16452
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis and In situ hybridization
|
|
ISCN
|
Human/hamster somatic cell hybrid retaining a human del(18)(pter>q21.3:) in 92% of cells
|
|
Remarks
|
|
| Remarks |
Line JH458; produced by the fusion of human cell line GM16451 (JL359) with karyotype 46,XY,del(18)(q21.3), with Chinese hamster cell line UCW206; grown at 39C; retains a human del(18)(pter>q21.3:) in 92% of the cells; the breakpoint was refined to 18q21.32 by molecular analysis |
| Mahr RN, Moberg PJ, Overhauser J, Strathdee G, Kamholz J, Loevner LA, Campbell H, Zackai EH, Reber ME, Mozley DP, Brown L, Turetsky BI, Shapiro RM, Neuropsychiatry of 18q- syndrome. Am J Med Genet67(2):172-8 1996 |
| PubMed ID: 8723044 |
| Split Ratio |
1:8 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|