GM16490
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 4 SOMATIC CELL HYBRIDS
Repository
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NIGMS Human Genetic Cell Repository
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Subcollection |
Hybrids |
Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/hamster somatic cell hybrid retaining a human der(18)t(4;18)(q31.1;q23) in 100% of cells and other human chromosomes
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Remarks
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IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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Remarks |
Line JH347; produced by the fusion of human cell line GM16434 (JL203) with karyotype 46,XY,der(18)t(4;18)(q31.1;q23), with Chinese hamster cell line UCW206; grown at 39C; retains a human der(18)t(4;18)(q31.1;q23) in 100% of the cells and human chromosomes 3, 4, 9, and 15 |
Mewar R, Kline AD, Jackson L, Overhauser J, Confirmation of a cryptic unbalanced translocation using whole chromosome fluorescence in situ hybridization. Am J Med Genet44(4):477-81 1992 |
PubMed ID: 1442891 |
Split Ratio |
1:11 |
Temperature |
39 C |
Percent CO2 |
8% |
Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
Serum |
10% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
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