GM16589
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 5 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
Human/hamster somatic cell hybrid retaining a human del(5)(p15.3p14.2) derived from an ins(16;5)(q22;p15.3p14.2) in 100% of cells
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line JH508; produced by the fusion of human lymphoblast line JL442 (GM16588), a mother, with karyotype 46,XX,ins(16;5)(q22;p14.2-p15.3) , of a Cri-du-Chat syndrome child and a Chinese hamster line; grown in culture medium containing 0.2mM proline and grown at 39C; retains a human del(5)(p15.3p14.2) in 100% of cells |
| Marinescu RC, Mamunes P, Kline AD, Schmidt J, Rojas K, Overhauser J, Variability in a family with an insertion involving 5p. Am J Med Genet86(3):258-63 1999 |
| PubMed ID: 10482876 |
| Split Ratio |
1:8 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
|
|