GM16599
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 5 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
Human/hamster somatic cell hybrid retaining a human del(5)(p15.2p13) in 96% of cells and human chromosomes 4, 8, 10, 13, 14, 16, 20, 21, and 22
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line JH164; produced by the fusion of human lymphoblast line JL88 (GM16598), from a Cri-du-Chat syndrome patient with karyotype 46,XY/46,XY,del(5)(p15.2-p13), with a Chinese hamster line; grown in culture medium containing 0.2mM proline and grown at 39C; retains a human del(5)(p15.2p13) in 96% of cells and other human chromosomes |
| Goodart SA, Simmons AD, Grady D, Rojas K, Moyzis RK, Lovett M, Overhauser J, A yeast artificial chromosome contig of the critical region for cri-du-chat syndrome. Genomics24(1):63-8 1994 |
| PubMed ID: 7896290 |
| Split Ratio |
1:12 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
|
|