GM19685
LCL from B-Lymphocyte
Description:
MEXICAN ANCESTRY IN LOS ANGELES, CALIFORNIA, USA
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Repository
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NHGRI Sample Repository for Human Genetic Research
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| Subcollection |
NHGRI Sample Repository for Human Genetic Research |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Country of Origin
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USA
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Family Member
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3
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Relation to Proband
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father
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
At least three out of four grandparents were born in Mexico; sample collected from an individual living in the Los Angeles, California metropolitan area |
| Zimmerman Zuckerman E, Thompson JA, Schneider AR, Campion MB, Johns JJ, Stier TJ, Peterson LM, Ward AM, Blommel JH, Gnanaolivu RD, Lauer KP, Sivasankaran G, Balan J, Dasari S, Sakai Y, Marcou CA, Zheng G, Halling KC, Shen W, Viswanatha DS, Niu Z, Automation of hybridization and capture based next generation sequencing library preparation requires reduction of on-deck bead binding and heated wash temperatures SLAS technology: 2022 |
| PubMed ID: 35058215 |
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| Numanagic I, Malikic S, Ford M, Qin X, Toji L, Radovich M, Skaar TC, Pratt VM, Berger B, Scherer S, Sahinalp SC, Allelic decomposition and exact genotyping of highly polymorphic and structurally variant genes Nature communications9:828 2017 |
| PubMed ID: 29483503 |
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| Shi W, Massaia A, Louzada S, Banerjee R, Hallast P, Chen Y, Bergström A, Gu Y, Leonard S, Quail MA, Ayub Q, Yang F, Tyler-Smith C, Xue Y, Copy number variation arising from gene conversion on the human Y chromosome Human genetics9:828 2017 |
| PubMed ID: 29209947 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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