GM22251
Keratinocyte from Skin, Foreskin
Description:
MULTIPLE CELL TYPES FROM SAME SUBJECT - FORESKIN
APPARENTLY HEALTHY INDIVIDUAL
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Apparently Healthy Collection |
|
Biopsy Source
|
Foreskin
|
|
Cell Type
|
Keratinocyte
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Keratinocyte from Skin, Foreskin
|
|
Race
|
White
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XY
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
26 |
| PDL at Freeze |
1.96 |
| Passage Frozen |
1 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Foreskin keratinocyte cell culture; this culture tested 95% positive for keratinocyte cell-specific cytokeratin (immunocytochemistry assay); other cultures from this same donor are GM22252 (fibroblast) and GM22250 (melanocyte). Must ship frozen. |
| Shirley MD, Baugher JD, Stevens EL, Tang Z, Gerry N, Beiswanger CM, Berlin DS, Pevsner J, Chromosomal variation in lymphoblastoid cell lines Human mutation33:1075-86 2011 |
| PubMed ID: 22374857 |
| PDL at Senescence |
26 |
| Cumulative PDL at Freeze |
1.96 |
| Passage Frozen |
1 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
CnT-07 Epidermal Progenitor Cell Medium |
| Serum |
0% none |
| Substrate |
Collagen IV-coated |
| Supplement |
- |
|
|