GM22254
Keratinocyte from Skin, Foreskin
Description:
MULTIPLE CELL TYPES FROM SAME SUBJECT - FORESKIN
APPARENTLY HEALTHY INDIVIDUAL
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Apparently Healthy Collection |
Biopsy Source
|
Foreskin
|
Cell Type
|
Keratinocyte
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Keratinocyte from Skin, Foreskin
|
Race
|
Hispanic/Latino
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
46,XY
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Senescence |
26 |
PDL at Freeze |
4.15 |
Passage Frozen |
2 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
|
Remarks |
Foreskin keratinocyte cell culture; this culture tested 99% positive for keratinocyte cell-specific cytokeratin (immunocytochemistry assay); other cultures from this same donor are GM22255 (fibroblast) and GM22253 (melanocyte). Must ship frozen. |
Shirley MD, Baugher JD, Stevens EL, Tang Z, Gerry N, Beiswanger CM, Berlin DS, Pevsner J, Chromosomal variation in lymphoblastoid cell lines Human mutation33:1075-86 2011 |
PubMed ID: 22374857 |
PDL at Senescence |
26 |
Cumulative PDL at Freeze |
7.25 |
Passage Frozen |
2 |
Split Ratio |
1:5 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
CnT-07 Epidermal Progenitor Cell Medium |
Serum |
0% none |
Substrate |
Collagen IV-coated |
Supplement |
- |
|
|