GM22254
Keratinocyte from Skin, Foreskin
Description:
MULTIPLE CELL TYPES FROM SAME SUBJECT - FORESKIN
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Keratinocyte
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Keratinocyte from Skin, Foreskin
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Race
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Hispanic/Latino
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Family Member
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1
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
26 |
| PDL at Freeze |
4.15 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Foreskin keratinocyte cell culture; this culture tested 99% positive for keratinocyte cell-specific cytokeratin (immunocytochemistry assay); other cultures from this same donor are GM22255 (fibroblast) and GM22253 (melanocyte). Must ship frozen. |
| Shirley MD, Baugher JD, Stevens EL, Tang Z, Gerry N, Beiswanger CM, Berlin DS, Pevsner J, Chromosomal variation in lymphoblastoid cell lines Human mutation33:1075-86 2011 |
| PubMed ID: 22374857 |
| PDL at Senescence |
26 |
| Cumulative PDL at Freeze |
7.25 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
CnT-07 Epidermal Progenitor Cell Medium |
| Serum |
0% none |
| Substrate |
Collagen IV-coated |
| Supplement |
- |
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