GM22263
Fibroblast from Skin, Foreskin
Description:
MULTIPLE CELL TYPES FROM SAME SUBJECT - FORESKIN
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
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Biopsy Source
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Foreskin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Foreskin
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Race
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Hispanic/Latino
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Family Member
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1
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
29 |
| PDL at Freeze |
2.84 |
| Passage Frozen |
1 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Foreskin fibroblast cell culture; other cultures from this same donor are GM22261 (melanocyte) and GM22262 (keratinocyte) |
| Shirley MD, Baugher JD, Stevens EL, Tang Z, Gerry N, Beiswanger CM, Berlin DS, Pevsner J, Chromosomal variation in lymphoblastoid cell lines Human mutation33:1075-86 2011 |
| PubMed ID: 22374857 |
| PDL at Senescence |
29 |
| Cumulative PDL at Freeze |
2.84 |
| Passage Frozen |
1 |
| Split Ratio |
1:20 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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