GM22575
Melanocyte from Skin, Foreskin
Description:
MULTIPLE CELL TYPES FROM SAME SUBJECT - FORESKIN
APPARENTLY HEALTHY INDIVIDUAL
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Apparently Healthy Collection |
|
Biopsy Source
|
Foreskin
|
|
Cell Type
|
Melanocyte
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Melanocyte from Skin, Foreskin
|
|
Race
|
Black/African American
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
45,XY,-9[2]/46,XY[48]
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Senescence |
30 |
| PDL at Freeze |
3.25 |
| Passage Frozen |
1 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
| |
| Remarks |
Foreskin melanocyte cell culture; this culture tested 99% positive for melanocyte cell-specific gp100 (immunocytochemistry assay); another culture from this same donor is GM22577 (fibroblast). Must ship frozen. |
| PDL at Senescence |
30 |
| Cumulative PDL at Freeze |
3.25 |
| Passage Frozen |
1 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Melanocyte Growth Medium |
| Serum |
0% none |
| Substrate |
Collagen IV-coated |
| Supplement |
- |
|
|