GM23760
iPSC from Fibroblast
Description:
SCHIZOPHRENIA; SCZD
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Lentiviral)
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Sample Source
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iPSC from Fibroblast
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Race
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White
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Ethnicity
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JEWISH/SCANDANAVIAN
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Family Member
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2
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
24 |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remarks |
Induced pluripotent stem cell culture derived from GM01792 Fibroblast; see GM01793 Lymphoid; episodes of agitation, delusions of persecution, and fear of assassination; father also affected. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc.. |
| Assis-de-Lemos G, Monteiro J, Oliveira-Valença VM, Melo GA, Reis RAM, Rehen SK, Silveira MS, Galina A, Dopamine signaling impairs ROS modulation by mitochondrial hexokinase in human neural progenitor cells Bioscience reports41: 2021 |
| PubMed ID: 34821365 |
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| Casas BS, Vitória G, do Costa MN, Madeiro da Costa R, Trindade P, Maciel R, Navarrete N, Rehen SK, Palma V, hiPSC-derived neural stem cells from patients with schizophrenia induce an impaired angiogenesis Translational psychiatry8:48 2017 |
| PubMed ID: 29467462 |
| Passage Frozen |
24 |
| Split Ratio |
1:8 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Knockout DMEM |
| Serum |
20% Knock-out Serum Replacement |
| Substrate |
Gelatin + Feeder Layer |
| Supplement |
Basic Fibroblast Growth Factor 50 ug/ml |
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