GM50060
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 5 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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ISCN
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Human/hamster somatic cell hybrid retaining a human del(5)(p13) in 88% of cells and 8, 14, and 18
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line JH141; produced by the fusion of human lymphoblasts from a patient with Cri-du-Chat syndrome, del(5)(p13), with Chinese hamster line UCW56 cells; grown in culture medium with 0.2mM proline at 39C; this hybrid retains a human del(5)(p13) in 88% of cells and a human chromosome 8, 14, and 18 |
| Overhauser J, Huang X, Gersh M, Wilson W, McMahon J, Bengtsson U, Rojas K, Meyer M, Wasmuth JJ, Molecular and phenotypic mapping of the short arm of chromosome 5: sublocalization of the critical region for the cri-du-chat syndrome. Hum Mol Genet3:247-52 1994 |
| PubMed ID: 8004090 |
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| Keppen LD, Gollin SM, Edwards D, Sawyer J, Wilson W, Overhauser J, Clinical phenotype and molecular analysis of a three-generation family with an interstitial deletion of the short arm of chromosome 5. Am J Med Genet44:356-60 1992 |
| PubMed ID: 1488985 |
| Split Ratio |
1:10 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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