GM50072
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 5 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
Human/hamster somatic cell hybrid retaining a human del(5)(p14.2) in 100% of cells and a human 22
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line JH246; produced by the fusion of human lymphoblasts, GM14126, from a patient with Cri-du-Chat syndrome with karyotype 46,XX,del(5)(p14), with Chinese hamster line UCW56 cells; grown in culture medium with 0.2mM proline at 39C; this hybrid retains a human del(5)(p14.2) in 100% of cells and a human 22 in 88% |
| Marinescu RC, Johnson EI, Dykens EM, Hodapp RM, Overhauser J, No relationship between the size of the deletion and the level of developmental delay in cri-du-chat syndrome. Am J Med Genet86(1):66-70 1999 |
| PubMed ID: 10440832 |
| |
| Overhauser J, Huang X, Gersh M, Wilson W, McMahon J, Bengtsson U, Rojas K, Meyer M, Wasmuth JJ, Molecular and phenotypic mapping of the short arm of chromosome 5: sublocalization of the critical region for the cri-du-chat syndrome. Hum Mol Genet3:247-52 1994 |
| PubMed ID: 8004090 |
| Split Ratio |
1:8 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
|
|