GM50079
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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ISCN
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Human/hamster somatic cell hybrid retaining a human i(18)(q10) in 80% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line JH312; produced by the fusion of human lymphoblasts, GM50121, from a patient with 18q- syndrome with karyotype 46,XY,i(18)(q10), with a Chinese hamster line UCW206; grown at 39C; retains a human i(18)(q10) in 80% of cells |
| Overhauser J, Mitchell HF, Zackai EH, Tick DB, Rojas K, Muenke M, Physical mapping of the holoprosencephaly critical region in 18p11.3. Am J Hum Genet57(5):1080-5 1995 |
| PubMed ID: 7485158 |
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| Mewar R, Harrison W, Overhauser J, Confirmation of isochromosome 18p using whole chromosome arm-specific fluorescence in situ hybridization. Cytogenet Cell Genet64(1):1-4 1993 |
| PubMed ID: 8508672 |
| Split Ratio |
1:10 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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