GM50081
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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Human/hamster somatic cell hybrid retaining a human del(18)(p11.1) in 96% of cells
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line JH357; produced by the fusion of human lymphoblasts, GM50126, from a patient with holoprosencephaly with karyotype 46,XX,del(18)(p11.1), with a Chinese hamster line UCW206; grown at 39C; retains a human del(18)(p11.1) in 96% of cells and a chromosome with the appearance of an i(18q) was seen in 4% |
| Overhauser J, Mitchell HF, Zackai EH, Tick DB, Rojas K, Muenke M, Physical mapping of the holoprosencephaly critical region in 18p11.3. Am J Hum Genet57(5):1080-5 1995 |
| PubMed ID: 7485158 |
| Split Ratio |
1:10 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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