GM50086
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
Human/hamster somatic cell hybrid retaining a human del(18)(p11.1) in 100% of cells and human chromosomes 3, 4, 5, 7, 8, 9, 11, 12, 13, 14, 15, 16, 17, 20, 21, and Y
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line JH410; produced by the fusion of human lymphoblasts from a patient with 18p- syndrome with karyotype del(18)(p11.1), with a Chinese hamster line; grown at 39C; retains a human del(18)(p11.1) in 100% of cells and other human chromosomes |
| Split Ratio |
1:7 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|