GM50099
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 18 SOMATIC CELL HYBRIDS
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Hybrids |
|
Cell Type
|
Somatic cell hybrid
|
|
Transformant
|
Untransformed
|
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
|
ISCN
|
Human/hamster somatic cell hybrid retaining a human del(18)(q23) in 68% of cells and a human chromosome 8
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
| |
| Remarks |
Line JH486; produced by the fusion of human lymphoblasts, GM50186, from a patient with 18q- syndrome with karyotype 46,XX,del(18)(q23), with a Chinese hamster line UCW206; grown at 39C; retains a human del(18)(q23) in 68% of cells and a human chromosome 8 in 76% of cells |
| Strathdee G, Sutherland R, Jonsson JJ, Sataloff R, Kohonen-Corish M, Grady D, Overhauser J, Molecular characterization of patients with 18q23 deletions. Am J Hum Genet60(4):860-8 1997 |
| PubMed ID: 9106532 |
| Split Ratio |
1:8 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|