GM50106
Somatic cell hybrid
Description:
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 5 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/hamster somatic cell hybrid retaining a human del(5)(p15.2) in 100% of cells and human 3, 16, 19, 20, and 22
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Line JH540; produced by the fusion of human lymphoblasts, GM50194, from a patient with Cri-du-Chat syndrome with a karyotype of 46,XX,del(5)(p15.2) with Chinese hamster line UCW56 cells; grown in culture medium with 0.2mM proline at 39C; this hybrid retains a human del(5)(p15.2) in 100% of cells and human 3, 16, 19, 20, and 22 |
| Split Ratio |
1:8 |
| Temperature |
39 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Proline 0.2 mM |
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