GM00345
Fibroblast from Skin, Unspecified
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Mesocricetus auratus
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Common Name
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golden (or syrian) hamster
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis |
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| Remarks |
Line TG-2, HPRT deficient, selectable with 6-thioguanine, heteroploid, fibroblast culture |
| Pettenati MJ, Le Beau MM, Lemons RS, Shima EA, Kawasaki ES, Larson RA, Sherr CJ, Diaz MO, Rowley JD, Assignment of CSF-1 to 5q33.1: evidence for clustering of genes regulating hematopoiesis and for their involvement in the deletion of the long arm of chromosome 5 in myeloid disorders. Proc Natl Acad Sci U S A84:2970-4 1987 |
| PubMed ID: 3495006 |
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| Clynes MM, Smyth H, O'Kennedy R, Corrigan A, Separation of viable from nonviable cells using a discontinuous density gradient. In Vitro16:809-12 1980 |
| PubMed ID: 7409839 |
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| Tsukamoto K, Klein R, Hatanaka M, Insertion of muntjac gene segment into hamster cells by cell fusion. J Cell Physiol104:225-32 1980 |
| PubMed ID: 7410491 |
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| Nakamura H, Littlefield JW, Purification, properties, and synthesis of dihydrofolate reductase from wild type and methotrexate-resistant hamster cells. J Biol Chem247:179-87 1972 |
| PubMed ID: 5062896 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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