GM00562
Fibroblast from Gonad, Gonad
Description:
ANEUPLOID CHROMOSOME NUMBER - NON-TRISOMIC
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Chromosome Abnormalities |
Biopsy Source
|
Gonad
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Gonad
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Gonad, Gonad
|
Race
|
Black/African American
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis after cell line submission to CCR
|
ISCN
|
45,X[46]/46,XX[4]
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
Passage Frozen |
4 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Remarks |
4 cells 46,XX, 46 cells 45,X; tissue from left gonad; gonad revealed testicular rather than ovarian tissue; same patient. as GM00563 |
Passage Frozen |
4 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
- |
|
|