Description:
MYELOMA (PLASMACYTOMA) CELL LINES FOR HYBRIDOMAS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Animals |
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Cell Type
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Myeloma
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
BALB/C mouse myeloma; line P3X63NS1; HPRT deficient, produces Kappa, nonimmunoglobulin secreting; medium contains 4.5g glucose per liter |
| Barnstable CJ, Bodmer WF, Brown G, Galfre G, Milstein C, Williams AF, Ziegler A, Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell14:9-20 1978 |
| PubMed ID: 667938 |
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| Kohler G, Milstein C, Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion. Eur J Immunol6:511-9 1976 |
| PubMed ID: 825377 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
10% |
| Percent O2 |
AMBIENT |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Semi-suspension culture |
| Supplement |
- |
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