GM04679
LCL from B-Lymphocyte
Description:
BURKITT LYMPHOMA; BL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
dbGaP |
| Class |
Heritable Cancer Syndromes and other Cancers |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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Black/African American
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY,add(8)(q?),add(14)(q?)
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Line EB-3; G6PD type A; positive for Epstein Barr virus; negative for reverse transcriptase; 46,XY,mar(8),mar(14); 10% of cells show random chromosome loss; band 8q23 is absent; 14q+ is unidentified |
| Henle G, Henle W, Diehl V, Relation of Burkitt's tumor-associated herpes-ytpe virus to infectious mononucleosis. Proc Natl Acad Sci U S A59:94-101 1968 |
| PubMed ID: 5242134 |
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| Kohn G, Mellman WJ, Moorhead PS, Loftus J, Henle G, Involvement of C group chromosomes in five Burkitt lymphoma cell lines. J Natl Cancer Inst38:209-22 1967 |
| PubMed ID: 6019732 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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