GM06141
LCL from B-Lymphocyte
Description:
HUMAN ERYTHROLEUKEMIC CELL LINES
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Mutations of the Hemoglobin Loci |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by chromosome Analysis |
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| Remarks |
Line HEL; derived from the peripheral blood of a patient with Hodgkin's disease who later developed erythroleukemia; negative for EBVNA, immunoglobulins, and T-lymphoid cell surface antigens; produces mainly Hb Barts in response to hemin |
| Suedhoff T, Birckbichler PJ, Lee KN, Conway E, Patterson MK Jr, Differential expression of transglutaminase in human erythroleukemia cells in response to retinoic acid. Cancer Res50:7830-4 1990 |
| PubMed ID: 1979250 |
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| Huebner K, Isobe M, Croce CM, Golde DW, Kaufman SE, Gasson JC, The human gene encoding GM-CSF is at 5q21-q32, the chromosome region deleted in the 5q- anomaly. Science230:1282-5 1985 |
| PubMed ID: 2999978 |
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| Martin P, Papayannopoulou T, HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science216:1233-5 1982 |
| PubMed ID: 6177045 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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