Description:
TRANSLOCATED CHROMOSOME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Chromosome Abnormalities
dbGaP |
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Cell Type
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Fibroblast
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Transformant
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Untransformed
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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47,XX,+der(13)t(13;17)(q22;q25)mat.ish der(13)(D17S2200+).arr 13q11q22.3(17943627-76295102)x3,17q25.3(74352508-78643088)x3
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Species
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Homo sapiens
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Common Name
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Human
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| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase and Glucose-6-Phosphate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Cytogenetics |
Chromosome 13: DERIVATIVE CHROMOSOME Aneuploid Segment (+)13pter>13q22 |
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Chromosome 13: DERIVATIVE CHROMOSOME Aneuploid Segment (+)13pter>13q22 |
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Chromosome 13: DERIVATIVE CHROMOSOME Trisomic Segment 13pter>13q22 |
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Chromosome 17: DERIVATIVE CHROMOSOME Aneuploid Segment (+)17q25>17qter |
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Chromosome 17: DERIVATIVE CHROMOSOME Aneuploid Segment (+)17q25>17qter |
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Chromosome 17: DERIVATIVE CHROMOSOME Trisomic Segment 17q25>17qter |
| Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013 |
| PubMed ID: 23665875 |
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| Wagner T, Tommerup N, Wirth J, Leffers H, Zimmer J, Back E, Weissenbach J, Scherer G, A somatic cell hybrid panel for distal 17q: GDIA1 maps to 17q25.3. Cytogenet Cell Genet76:172-5 1997 |
| PubMed ID: 9186513 |
| Passage Frozen |
5 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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