GM07297
Somatic cell hybrid
Description:
ADDITIONAL HUMAN/CHINESE HAMSTER SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Family Member
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3
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Confirmation
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Karyotypic analysis and In situ hybridization
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ISCN
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Human/Chinese hamster somatic cell hybrid retaining human chromosomes X & #3
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - X CHROMOSOME |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for Xp22.3p22.2, DXS85, and Xq28, DXS15. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for Xp22.32, STS, Xq13.3, PGK1, Xq26, HPRT, and Xq28, G6PD. |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
DNA from this somatic cell hybrid gave negative results in Southern blot hybridization analyses with probes for Yp11.3 (also Xp22.32), DXYS20, and Yq12, DYZ1. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 1 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 1q32, REN, and a positive result with a probe for 1p36 (also 3p21), DNF15S1/S2. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 3 |
DNA from this somatic cell hybrid gave positive results in Southern blot hybridization analyses with probes for 3p25, RAF1, and 3q21, TF. PCR analysis of DNA from this somatic cell hybrid gave positive results with primers for 3p22-p21.33, GLB1, and 3q22-q24, D3S1764. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 5 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 5q31q32, ADRBR. |
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| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 21 |
DNA from this somatic cell hybrid gave a negative result in Southern blot hybridization analysis with a probe for 21q22.3, BCEI. |
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| Remarks |
Line #11.1.2.2; human/Chinese hamster lung cell line; selected in medium containing azaserine; retains only human chromosomes #3 & X; at passage 6, 88% of cells have the human X & 60% of cells have human chromosome #3 |
| Saito M, Helin K, Valentine MB, Griffith BB, Willman CL, Harlow E, Look AT, Amplification of the E2F1 transcription factor gene in the HEL erythroleukemia cell line. Genomics25:130-8 1995 |
| PubMed ID: 7774910 |
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| Hendershot LM, Valentine VA, Lee AS, Morris SW, Shapiro DN, Localization of the gene encoding human BiP/GRP78, the endoplasmic reticulum cognate of the HSP70 family, to chromosome 9q34. Genomics20:281-4 1994 |
| PubMed ID: 8020977 |
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| Huebner K, Kastury K, Druck T, Salcini AE, Lanfrancone L, Pelicci G, Lowenstein E, Li W, Park SH, Cannizzaro L, et al, Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2). Genomics22:281-7 1994 |
| PubMed ID: 7806213 |
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| Kastury K, Druck T, Huebner K, Barletta C, Acampora D, Simeone A, Faiella A, Boncinelli E, Chromosome locations of human EMX and OTX genes. Genomics22:41-5 1994 |
| PubMed ID: 7959790 |
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| Siden TS, Golembieski W, Kumlien J, Rohme D, Smith DI, Physical map of small cell lung cancer deletion region on short arm of human chromosome 3 (3p13-22) based on radiation fusion hybrid analysis. Somat Cell Mol Genet20:121-32 1994 |
| PubMed ID: 8009365 |
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| Howe JR, Lairmore TC, Veile R, Dou S, Wells SA Jr, Donis-Keller H, Development of a sequence-tagged site for the centromere of chromosome 10: its use in cytogenetic and physical mapping. Hum Genet91:199-204 1993 |
| PubMed ID: 8478002 |
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| Huebner K, Druck T, LaForgia S, Lasota J, Croce CM, Lanfrancone L, Donti E, Pengue G, La Mantia G, Pelicci PG, et al, Chromosomal localization of four human zinc finger cDNAs. Hum Genet91:217-22 1993 |
| PubMed ID: 8478004 |
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| LaForgia S, Lasota J, Latif F, Boghosian-Sell L, Kastury K, Ohta M, Druck T, Atchison L, Cannizzaro LA, Barnea G, et al, Detailed genetic and physical map of the 3p chromosome region surrounding the familial renal cell carcinoma chromosome translocation, t(3;8)(p14.2;q24.1). Cancer Res53:3118-24 1993 |
| PubMed ID: 8319219 |
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| Vooijs M, Yu LC, Tkachuk D, Pinkel D, Johnson D, Gray JW, Libraries for each human chromosome, constructed from sorter-enriched chromosomes by using linker-adaptor PCR. Am J Hum Genet52:586-97 1993 |
| PubMed ID: 8447324 |
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| Morris SW, Nelson N, Valentine MB, Shapiro DN, Look AT, Kozlosky CJ, Beckmann MP, Cerretti DP, Assignment of the genes encoding human interleukin-8 receptor types 1 and 2 and an interleukin-8 receptor pseudogene to chromosome 2q35. Genomics14:685-91 1992 |
| PubMed ID: 1427896 |
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| Rettig WJ, Grzeschik KH, Yenamandra AK, Garcia E, Old LJ, Definition of selectable cell surface markers for human chromosomes and chromosome segments in rodent-human hybrids. Somat Cell Mol Genet14:223-31 1988 |
| PubMed ID: 3163425 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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