Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection |
| Class |
Disorders of the Nervous System |
|
Cell Type
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Fibroblast
|
|
Transformant
|
Untransformed
|
|
Race
|
White
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
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Clinical summary/Case history
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|
Species
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Homo sapiens
|
|
Common Name
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Human
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|
Remarks
|
|
| PDL at Freeze |
7.03 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| Remarks |
See GM7544 Lymphoid |
| Wilson E, Knudson W, Newell-Litwa K, Hyaluronan regulates synapse formation and function in developing neural networks Scientific reports10:16459 2020 |
| PubMed ID: 33020512 |
| |
| Porterfield V, Khan SS, Foff EP, Koseoglu MM, Blanco IK, Jayaraman S, Lien E, McConnell MJ, Bloom GS, Lazo JS, Sharlow ER, A three-dimensional dementia model reveals spontaneous cell cycle re-entry and a senescence-associated secretory phenotype Neurobiology of aging90:125-134 2019 |
| PubMed ID: 32184029 |
| |
| Wilson E, Rudisill T, Kirk B, Johnson C, Kemper P, Newell-Litwa K, Cytoskeletal regulation of synaptogenesis in a model of human fetal brain development Journal of neuroscience research90:125-134 2019 |
| PubMed ID: 32713041 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
|
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